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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has been observed in several tumors, which leads to chromosome instability, and so may well lead to tumorigenesis. This protein was located to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a critical job in endorsing mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up documented but comprehensive nucleotide sequence information is not nevertheless accessible.

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Blocking peptides are peptides that bind specially to the goal antibody and block antibody binding. These peptide normally incorporates the epitope regarded from the antibody.

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This guidebook outlines further more feasible optimization of cellular and immunoassay parameters to be sure the absolute best success are...

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This guide outlines additional achievable optimization of cellular and immunoassay parameters to ensure the best possible results are...

Antibodies sure to the blocking peptide no more bind on the epitope about the target protein. By comparing the staining from the blocked antibody as opposed to the antibody on your own, one can see which staining is unique.

Focus on Data Cyclin E belongs towards the highly conserved cyclin spouse and children, whose customers are characterized by a extraordinary periodicity in protein abundance throughout the mobile cycle. Cyclins operate as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation patterns which add towards the temporal coordination of every mitotic event. Cyclin E varieties a complex with and features to be a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S changeover. This protein accumulates on the G1-S section boundary and is degraded as cells progress by S section.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...

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